Be
a Part of the Biotechnology
Revolution To
The Student Now,
imagine being a part of this revolution. Imagine
working on a team that constructs a vaccine for the
HIV virus, which causes AIDS. Imagine working in a
company that produces a high-protein peanut which
can be grown in drought conditions in third-world
countries. Imagine being in the laboratory that
develops a bacterium, which digests petroleum from
oil spills. Imagine the sense of accomplishment and
gratification you'd feel as a contributor to these
breakthroughs. Imagine how you can help improve the
quality of life for your friends and
family. You
can be part of the Biotechnology Revolution.
Thousands of employees are needed to staff the
hundreds of new biotech companies starting up every
year. These fledgling companies need scientists and
lab technicians to complete research and
development on potential products. Whether
you aspire to work in a lab, on research and
development, or a career in the financial or
business end of biotechnology there is a surplus of
jobs to entice people of all interests. No matter
what your position, be it entry-level or
Vice-President, when a product is released that you
have had some part in developing, a wonderful
feeling of satisfaction arises. The
purpose of this text is to provide the reader with
an introduction to the many skills necessary to
work in the field of biotechnology. The emphasis
lies in the areas of research and production. No
matter where an individual's interest lies, or what
position an employee has (science or business),
understanding the tools and techniques of basic
biotechnology research will result in greater
ability to work effectively on a team, bringing a
profit-making product to market. •
Click
here to get more information about ordering the
book from the publisher, EMC-Paradigm
Publishing. What
is Biotechnology? The
Raw Materials of
Biotechnology Basic
Skills of the Biotechnology
Workplace Introduction
to the Study of DNA
Molecules Introduction
to the Study of Protein
Molecules Finding
a Potential Biotechnology
Product Spectrophotometers
and Assays for Biotechnology
Products Modeling
the Production of a Biotechnology
Product Bringing
a Biotechnology Product to
Market Introduction
to Plant Biotechnology Biotechnology
in Agriculture Biotechnology
in Medicine Making
DNA Molecules Advanced
Biotechnology
Techniques Chapter
1 What
is Biotechnology? 1.1 What
is Biotechnology? 1.2 Doing
Biotechnology: Scientific Methodology in a
Research Facility 1.3 The
Variety of Biotechnology
Products 1.4 How
Companies Pick Potential
Products 1.5 Who
does Biotechnology? Careers in the
Biotechnology Industry 1.6 Biotechnology
with a Conscience - Bioethics Chapter
2 The
Raw Materials of
Biotechnology 2.1 The
Raw Materials of Biotechnology 2.2 Cellular
Organization and Processes 2.3 The
Molecules of Cells 2.4 The
"New" Biotechnology - Manipulating
Molecules Chapter
3 Basic
Skills of the Biotechnology
Workplace 3.1 Measuring
Instruments in a Biotechnology
Facility 3.2 Making
Solutions 3.3 Solutions
of a Given Mass/Volume
Concentration 3.4 Solutions
of a Given % Mass/Volume
Concentration 3.5 Solutions
of Differing Molar
Concentrations 3.6 Dilutions
of Concentrated Solutions Chapter
4 Introduction
to the Studying of DNA
Molecules 4.1 DNA
Structure and Function 4.2 Sources
of DNA 4.3 Isolating
and Manipulating DNA 4.4 Using
Gel Electrophoresis to Study
Molecules Chapter
5 Introduction
to the Studying of Protein Molecules
5.1 The
Structure and Function of
Proteins 5.2 The
Production of Proteins 5.3 Enzymes:
Protein Catalysts 5.4 Studying
Proteins 5.5 Why
Study Proteins? Chapter
6 Finding
a Potential Biotechnology
Product 6.1 Sources
of Potential Products 6.2 The
Use of Assays 6.3 Looking
for Products in Nature 6.4 Studying
Plant Proteins as Possible
Products 6.5 Producing
rDNA Protein Products Chapter
7 Spectrophotometers
and Assays for Biotechnology
Products 7.1 Using
the Spectrophotometer to Detect
Molecules 7.2 roduction
to pH 7.3 Buffers 7.4 Using
the Spectrophotometers to Measure Protein
Concentration Chapter
8 Modeling
the Production of a Biotechnology
Product 8.1 Producing
a Genetically Engineered
Product 8.2 Transforming
Cells 8.3 After
Transformation 8.4 Fermentation,
Manufacturing, and GMP 8.5 Retrieving
Plasmids after Transformation Chapter
9 Bringing
a Biotechnology Product to
Market 9.1 Harvesting
a Protein Product 9.2 Using
Chromatography to Study and Separate
Molecules 9.3 Column
Chromatography 9.4 Product
Quality Control 9.5 Marketing
and Sales Chapter
10 Introduction
to Plant Biotechnology 10.1 Introduction
to Plant Propagation 10.2 Basic
Plant Anatomy 10.3 Plant
Growth, Structure, and Function 10.4 Introduction
to Plant Breeding 10.5 Statistical
Analysis of Data Chapter
11 Biotechnology
in Agriculture 11.1 Cloning
Plants 11.2 Cloning
by Plant Tissue Culture 11.3 New
Applications of Biotechnology in
Agriculture and Horticulture 11.4 Isolating
DNA from Plant Cells Chapter
12 Biotechnology
in Medicine 12.1 Drug
Discovery 12.2 Creating
Pharmaceuticals through Combinatorial
Chemistry 12.3 Creating
Pharmaceutical through Peptide or DNA
Synthesis 12.4 Creating
Pharmaceutical by Protein/Antibody
Engineering Chapter
13 Making
DNA Molecules 13.1 Making
DNA Molecules - DNA Synthesis 13.2 DNA
Synthesis Products 13.3 Polymerase
Chain Reaction - PCR 13.4 Applications
of PCR Technology Chapter
14 Advanced
Biotechnology Topics 14.1 Advanced
DNA Topics - DNA Sequencing 14.2 Advanced
DNA Studies - Genomics 14.3 Advanced
Protein Studies 14.4 Other
Advances in Biotechnology To
the Student: Be a Part of the
Biotechnology Revolution Chapter
1
Introduction
to Biotechnology
Methodologies 1a How
to Set Up a Legal Scientific
Notebook/Documentation 1b Lab
Safety/Inventory of the Biotechnology
Laboratory 1c The
Evolution of Cheese Production Chapter
2
Basic
Biology for the
Biotechnician 2a Dissecting
a Cell and Examining its
Components 2b The
Characteristics of Model
Organisms 2c Using
a Compound Light Microscope to Study
Cells 2d Making
Microscopic Measurements 2e Variations
in the Structure and Properties of
Carbohydrates 2f How
Molecular Structure Is Affected by
Environmental Change Chapter
3
Basic
Chemistry for the
Biotechnician 3a Measuring
Small Volumes in Biotech 3b Measuring
Very Small Volumes in Biotech 3c Measuring
Mass 3c.1 Metric
Instrument and Conversion Review
Sheet 3d Checking
the Accuracy of a Micropipet Using a
Balance 3e Making
Solutions of Differing Mass/Volume
Concentrations 3e.1 Making
Solutions Review Sheet #1 3f Making
Solutions of Differing % Mass/Volume
Concentrations 3f.1 Making
Solutions Review Sheet #2 3g Making
Solutions of Differing Molar
Concentrations 3g.1 Making
Solutions Review Sheet #3 3g.2 Making
Solutions Review Sheet #4 3h Making
Dilutions of Concentrated
Solutions Chapter
4 DNA
Isolation and Analysis 4a Making
Solutions for DNA Isolation 4b Pulling
DNA out of Solutions/DNA
Spooling 4c Testing
for the Presence of DNA, RNA, and Protein
in DNA Extracts 4d EtBr
Dot Test - A Quick Test for DNA in
Samples 4e Making
Media for Bacteria Cell Culture (Media
Prep) 4f Sterile
Technique and Pouring Plates 4g Bacteria
Cell Culture 4h DNA
Extraction from Bacteria 4i Making
Agarose Gels 4j Using
Gel Electrophoresis to Study DNA
Molecules Chapter
5
Protein
Isolation and Analysis 5a The
Specificity of Antibody
Proteins 5b The
Action of Enzymes on Juice
Production 5c Developing
an Assay for Protease Activity 5d Testing
for the Presence of Protein in
Solution 5e Preparing
Proteins for Analysis by Vertical Gel
Electrophoresis 5f Characterizing
Proteins by PAGE 5g Separating
and Identifying Proteins via
SDS-PAGE Chapter
6
Assay
Development 6a How
Do You Know When You Have Amylase?
(Experimental Design) 6b Assaying
for Starch and Sugar 6c Assaying
for Amylase Activity 6d Testing
Plant Substances as Potential
Medicines 6e Searching
for Native Amylase-Producing
Bacteria 6f Isolating
Horseradish Peroxidase from Radish
Root 6g Testing
for Peroxidase Activity using
TMB Chapter
7
Using
the Spectrophotometer for Protein
Assays 7a Learning
to Use the Spectrophotometer 7b Using
the Spectrophotometer to Study
Molecules 7c Measuring
the pH of Different Solutions 7d Making
an Appropriate Buffer for Protein Storage
and Activity 7e Demonstration
of Buffer Efficacy 7f Using
the Spectrophotometer to Study the
Protein, Amylase 7g Determining
the Concentration of Amylase in
Solution 7h Comparing
Assay Techniques - BCA vs
Bradford 7i Using
the UV Spec to Study Colorless
Proteins Chapter
8
Recombinant
Protein Production 8a Restriction
Analysis of the Lambda Phage DNA
Sequence 8b Restriction
Digestion to Verify the pAmylase
Plasmid 8c Transformation
of E. coli with pAmylase 8d Growing
and Monitoring Bacterial
Cultures 8e Scaling-Up
E. coli Cultures for Amylase
Production 8f Mini
Preparation of pAmy Using Lysozyme
Digestion 8g Alkaline
Cell Lysis Mini Preparation of
pAmyase Chapter
9
Protein
Product Purification and
Analysis 9a Harvesting
Amylase from Broth Culture 9b Dialysis
of Proteins into Different
Buffers 9c Using
Ion-Exchange Chromatography to Separate
Proteins 9d Using
Ion-Exchange Chromatography to Purify
Amylase from Scale-up Broth 9e Identification
of Amylase after Column Chromatography
using PAGE Chapter
10 Plant
Breeding 10a Flower
Morphology/Dissection 10b Seed
Morphology/Dissection 10c Seed
Germination Lab - Which Came
First? 10d Plant
Breeding Practice Crosses
Worksheet 10e Wisconsin
Fast Plant Breeding - A Model Organism for
Breeding 10f How
Can You Tell if Data is Good
Enough? Chapter
11 Plant
Cloning 11a Asexual
Plant Propagation through Leaf and Stem
Cuttings 11b Asexual
Plant Propagation through
Runners 11c The
Effect of Hormone Concentration on
Propagation 11d Cloning
African Violets 11e Using
Hydroponics to Improve
Fertilizers 11f Developing
an Extraction Protocol for Spoolable DNA
from Plant Cells 11g Using
Commercial Kits for DNA Extractions from
Cells 11h Determining
the Presence of DNA in Spinach
Extractions 11i Determining
the Purity and Concentration of
DNA 11j Transformation
of Arabidopsis 11k Confirmation
of Plant Genetic Engineering through
PCR Chapter
12 Obtaining
Molecules of Pharmaceutical
Interest
by Ellyn Daugherty
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Biotechnology: Science for the New
Millennuim
Take a Tour through
Biotechnology: Science for the New
Millennium
(available in PowerPoint and
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Imagine
a world where babies are born without defects,
criminals are identified within minutes, diseases
are identified and treated right in your own home,
and there is enough food to feed everyone. The
makings of a science fiction novel you might say,
but this "fiction" is closer than you think. Every
day new advances in the field of biotechnology
bring the possibilities of curing disease, wiping
out hunger, and improving the quality of life for
all people closer to a reality. Some say we are
undergoing a biotechnology revolution where the
discoveries and products made are revolutionizing
the way we live.
They
also need workers to perform manufacturing and
packaging. Since testing has to be done on all
products, employees are needed to design, run, and
document these tests. Companies also require
nonscientific support staff including those in
marketing, finance, and law. Human Resource
specialists are needed to ensure that employees are
well compensated. Public Relations people and those
in Corporate Communications keep the public
informed about product development and keep
employees aware of recent news on related
items.
• Click
here for Sargent-Welch Equipment and
Materials support.
Science for the New Millennium, First
Edition
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